research assistant

  • Volet de programme : Jeunesse Canada au travail dans les deux langues officielles
  • Titre de l'emploi : research assistant
  • Nom de l’organisme : Institut atlantique de recherche sur le cancer
  • Lieu de l’emploi : Moncton , Nouveau-Brunswick
  • Durée de l'emploi : 2018-05-07 à 2018-08-24 (10 semaines)
  • Salaire horaire : 13,50 $

The Atlantic Cancer Research Institute (ACRI) is a non-profit organization founded in late 1998 and housed at the Dr. Georges-L.-Dumont University Hospital Centre in Moncton. Thanks to its unique expertise, ACRI has become a true centre of excellence in cancer research. Scientists are working with partners in Canada and around the world to discover the genetic origins of cancer and pursuing three main areas of development: early detection of disease, enhanced diagnosis, and targeted treatment. The project:

- Cancer cells undergo spontaneous mutations to impart a survival advantage over other non-cancerous cells

- These mutations, while originally providing a benefit, can also be exploited for therapeutic advantage in the case of synthetic lethal interactions

- A genome wide screen for synthetic lethal targets provided 10 targets that require additional experimentation. These targets are broken into 2 classes; internal and external proteins

- We aim to have a student help us characterize the internet targets generated from this screen


Six putative synthetic lethal targets were found. The roles of these proteins varies but most are related to growth and proliferation either through a signal cascade or activity toward protein translation. Of these 6, 3 have commercially available inhibitors of function that allow for a quick screen to see if loss in activity causes cell death in cancer cells while the other 3 require more intense analysis. We aim to perform genetic analysis of the remaining 3 protein targets to determine if they are, in fact, synthetic lethal in lung cancer cell lines. A protein that is found to be a lethal target will further be characterized and assayed for potential therapeutic viability.

Role of a student on this project:

We require a student to perform the basic level experiments that will confirm if these proteins are actually synthetic lethal targets in cancer cells. This includes analysis of the differences in cell viability between cancer and non-cancer cells when these proteins are removed either via CRISPR or siRNA means. The student would undertake preparation tasks for assays such as flow cytometry and have an active role in protein determination via western blot. The student undertaking this role would also gain experience in cell culture and be called upon to perform individual experiments across many genetically unique lung cancer cell lines. A solid understanding of molecular biology and genetics will be provided to the student through this project while they will provide valuable primary data to the project while gaining that knowledge.

Qualifications: Completion of second year undergrad program in Biology / biochemistry / molecular biology

Wage: $13.50 / hour Interested students may apply by email: Deadline: April 6th, 2018

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